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KMID : 1094720150200020225
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 2 p.225 ~ p.233
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rational design and directed evolution of CYP102A1 (BM3) for regio-specific hydroxylation of isoflavone
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Ko Sung-Ghi
Yang Yung-Hun
Choi Kwon-Young
Kim Byung-Gee
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Abstract
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Isoflavonoids are a class of polycyclic aromatic hydrocarbon compounds originating from plants as secondary metabolites. Their oxidative metabolites such as ortho dihydroxyisoflavones (ODIs), where the ortho carbons of the hydroxyl groups are hydroxylated, have been reported to have a remarkably high antioxidant effect. Here, we report enzyme engineering of the cytochrome P450 enzyme CYP102A1 (BM3) for the synthesis of ODIs from isoflavonoids.
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KEYWORD
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cytochrome P450 monooxygenases, self-sufficient CYP102A1, isoflavone, ortho-specific hydroxylation, regioselectivity
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